Study of Conjugation in E.Coli

STUDY OF CONJUGATION IN E.coli AIM : To study the restore of bacterial conjugation through enthrall of factors,coding for antibiotic resistance. article of faith : From one bacterial attain(donor) to another strain(recipient) is a known particular(prenominal) that occurs in nature with objective of mixing the gene pool. In otherwise asexually reproducing organism, DNA wobble among bacteria is in different mediating ways: I. Transformation II. Transduction III. Conjugation Conjugation is the intimately widespread routine of transferring genetic material from one bacterial mobile phoneular phone to another. It is a process in which unidirectional transfer of DNA is arbitrate by conjugal plasmid DNA or conjugal transposons requiring cell to cell contacts. It was discovered by Lederberg and Tatum. Conjugation is best soundless by considering properties of F-factor, which is a low-spirited circular plasmid DNA, that poop replicate autonomously in the cell o r can shuffle into host chromosome and thus transfer host chromosomal marker. The man-to-man cells with integrated F-factor ar dislocated and allowed to form pure colonies. The resulting straincan transfer chromosomal marker at very high frequency and termed as Hfr strain. INTRODUCTION: The E.coli strain A & B referred to as parental strains.

give A carries an antibiotic patient of gene in F-plasmid coding for tetracycline, objet dart Strain B is detached of any F-factor, but carries streptomycin disgustful gene in its chromosome. consequently both the strains grows on strong suit containing tetracycline or streptomycin to which they are resistant ! on conjugating the 2 parental strains, the resulting culture go out be resistant to both the antibiotics, i.e. the conjugate sample when plate on a medium containing tetracycline or streptomycin willing survive suggesting that gene transfer has taken place from Strain A to B. save the parental strain when plated on the uniform medium does not survive as they are sore to either tetracycline or streptomycin. MATERIALS REQUIRED:...If you want to experience a mount essay, order it on our website: OrderCustomPaper.com

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